Human Glioma Cell Cultures.
HGCC is a biobanking effort to provide a panel of newly established and characterized GBM cell lines from GBM patient surgical samples, currently holding 48 cell lines. The HGCC cell lines and accompanying data are available as an open resource facilitating accurate modeling of GBM diversity for basic research and drug discovery.
Glioblastoma and GSCs
From patient to treatment.
The concept of glioma stem cells (GSCs) has attracted a lot of interest, and a glioma-initiating cell bearing stem cell characteristic has been proposed, with the ability to seed new tumors through the capacity to evade chemotherapy and irradiation.
Basic cancer research, including preclinical tumor models and testing of candidate drugs needs optimized in vitro models that better reflect the patient’s disease, including modeling of the cancer stem cell compartment. We have set up a biobanking effort to culture and characterize a new panel of cells, derived from GBM patient biopsies, based on a combination of two validated techniques for culturing GSCs (Xie et al. 2015). Here, we report the establishment, genomic characterization and in vivo validation of 48 patient-derived GBM cell lines cultured under stem cell conditions, and that they represent all four molecular GBM subtypes.
|Diagnosis||Number of patients||Mean age (span)||Male||Female||Number of cell lines||Cell line rate (%)|
|All glioma||101||60.7 (21-82)||56||45||54||47.4|
HGCC in Action
Drug Development and Characterization.
A number of studies have been published using HGCC. Here we summarize the cell lines used in each study.
|Xie et. al.|
|Kitambi et. al.|
|Wee et. al.|
|Kundu et. al.|
|Wee et. al.|
|Roy et. al.|
|Schmidt et. al.|
|Segerman et. al.|
|Jiang et. al.|
U3002, U3004, U3005, U3008, U3009, U3013, U3016, U3017, U3019, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3067, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3101, U3110, U3117, U3118, U3121, U3123
Cell 157,313-328 (2014)
U3013, U3047, U3024, U3017, U3031, U3037, U3086, U3054, U3065
PLoS One. 2014 Dec 22;9(12):e115698.
U3047, U3013, U3065, U3037, U3035, U3024, U3084, U3046, U3017
Eur J Pharmacol. 2014 Dec 12;748C:101-107.
Oncogene. 2013 Mar 25.
PLoS One. 2013;8(1):e54952. Epub 2013 Jan 25.
Mol Cancer Res. 2016 Aug 26.
Sci Rep. 2016 Jul 26;6:25956.
PLoS Genet. 2016 May;12(5):e1006000.
U3004, U3009, U3013, U3021, U3029, U3031, U3054
Oncotarget. 2015 Sep 15;6(27):23647-61.
U3016, U3047, U3117, U3065, U3060, U3062, U3101
U3013, U3017, U3024, U3037, U3065, U3084, U3002, U3004, U3005, U3009, U3020, U3035, U3008, U3016, U3021, U3027, U3031, U3046, U3029, U3028, U3033, U3039, U3047, U3085, U3019, U3034, U3056, U3082, U3086, U3117, U3053, U3054, U3060, U3073, U3078, U3088, U3051, U3062, U3068, U3110, U3118, U3129
U3017, U3065, U3117, U3118, U3121, U3167
U3002, U3005, U3008, U3009, U3013, U3017, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3093, U3101, U3102, U3104, U3110, U3117, U3118, U3121, U3123, U3129, U3137, U3140, U3146, U3155, U3164, U3167, U3169, U3172, U3173, U3175, U3180, U3189, U3198, U3202
Image AttributionsGlioblastoma MRI, Case courtesy of Dr Frank Gaillard, Radiopaedia.org. From the case Glioblastoma multiforme (GBM) with PNET component
Start page background: Intermediate magnification micrograph of a glioblastoma
Copyright © 2011 Nephron
Permission is granted to copy, distribute and/or modify this image under the terms of the GNU Free Documentation License Version 1.2 or any later version published by the Free Software Foundation.